Generation of three control iPS cell lines for sickle cell disease studies by reprogramming erythroblasts from individuals without hemoglobinopathies


Sickle cell disease (SCD) is one of the most prevalent and severe monogenetic disorders. Previously, we generated iPS cell lines from SCD patients. Here, we generated iPS cell lines from three age-, ethnicity- and gender-matched healthy individuals as control cell lines. Cell reprogramming was performed using erythroblasts expanded from PBMC by a non-integrative method. SCD-iPSC controls expressed pluripotency markers, presented a normal karyotype, were able to differentiate into the three germ layers in embryoid body spontaneous differentiation and confirmed to be integration-free. The cell lines generated here may be used as matched healthy controls for SCD studies. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Paredes BD, Martins GLS, Azevedo CM, Sampaio GLA, Nonaka CKV, Silva KND, Soares MBP, Santos RRD, Souza BSF
Journal Stem cell research
Publication Date 2019 Jul;38:101454
PubMed 31078962
DOI 10.1016/j.scr.2019.101454

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