Generation and characterization of iPS cell line (SYSUSHi002-A) from PBMCs of an AML patient with TP53 mutation
Summary
We have generated an iPSCs line (IPS-AML2-C3, SYSUSHi002-A) from AML cells of a 71-year-old male Acute Myeloid Leukaemia patient with TP53 gene mutation (TP53: c.824G > A, p.Cys275Tyr) using episomal plasmids encoding the factors OCT4, SOX2, KLF4, L-MYC and human miR-302. The IPS-AML2-C3 (SYSUSHi002-A) iPSC line displayed typical embryonic stem cell-like morphology, carried the TP53 gene mutation, expressed several pluripotent stem cell makers, retained normal karyotype (46, XY), and was capable of forming three germ layer cells in vitro. Copyright © 2023. Published by Elsevier B.V.
| Authors | Yuan L, Wang Y, Xie R, Lin D, Wang X |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2023 Dec;73:103230 |
| PubMed | 37890328 |
| DOI | 10.1016/j.scr.2023.103230 |