TMD145.E46.G1S

General

Cell Line

hPSCreg Name
CHOPi008-B
Alternative name(s)
TMD145.E46.G1S
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 27th October 2022
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Provider

Generator The Children's Hospital of Philadelphia (CHOP)

External Databases

Cellosaurus CVCL_C1NV
BioSamples SAMEA111510100
CLO CLO_0104117
Wikidata Q114310961

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Transient Abnormal Myelopoiesis Associated with Down Syndrome
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Transient Abnormal Myelopoiesis Associated with Down Syndrome
  • Transient Abnormal Myelopoiesis
  • Transient Abnormal Myelopoiesis (TAM)
  • Transient Myeloproliferative Disorder
  • Transient abnormal myelopoiesis
  • TAM
show more synonyms

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
47,XY+21

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA111510066

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Children's Hospital of Philadelphia, The Committees for the Protection of Human Subjects (IRB)
Approval number IRB 13-010038
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
Peripheral Blood
Blood drawn from a limb.
Synonyms
  • Peripheral Blood

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Medium Other medium:
Base medium: DMEM/F12
Main protein source:
Serum concentration: %

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
SOX17
Yes
FOXA2
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
CD41
Yes
GYPA
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
PAX6
Yes
FOXG1
Yes

Microbiology / Virus Screening

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage:

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
47,XY+21

Other Genotyping (Cell Line)