SA121
CEBe012-A
General#
Donor Information#
General Donor Information |
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Sex | male |
Phenotype and Disease related information (Donor) |
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Diseases | |
External Databases (Donor) |
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BioSamples | SAMEA6407438 |
Ethics#
Alternatives to consent are available? | Yes |
Alternatives to consent | |
Alternative consent approval number | |
How may genetic information associated with the cell line be accessed? |
hESC Derivation#
Date of derivation | 2002-04-29 |
Embryo stage | Blastula with ICM and Trophoblast |
PGD Embryo? |
No |
ZP removal technique | Enzymatic |
Cell isolation | Immunosurgery |
Cell seeding | Isolated ICM |
Culture Conditions#
Feeder cells |
mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040 |
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O2 Concentration | 20 % | |||||||||
CO2 Concentration | 5 % | |||||||||
Medium |
Other medium:
Base medium: DMEM/F12 with Glutamax
Main protein source: Anti-Human-Serum (Sigma) Serum concentration: % Supplements
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Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
Alkaline Phosphatase |
Yes |
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POU5F1 (OCT-4) |
Yes |
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SSEA-3 |
Yes |
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SSEA-4 |
Yes |
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TRA 1-60 |
Yes |
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TRA 1-81 |
Yes |
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SSEA-1 |
No |
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telomerase reverse transcriptase |
Yes |
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Differentiation Potency
Microbiology / Virus Screening |
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HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
46XY
Passage number: 35
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Other Genotyping (Cell Line) |
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