SA121

General#

Cell Line

hPSCreg Name CEBe012-A
Alternative name(s)
SA121
Cell line type Human embryonic stem cell (hESC)
Last update 19th November 2019
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Provider

Generator Cellartis AB Sweden (CEB)
Derivation country Sweden

External Databases

Cellosaurus CVCL_B296
BioSamples SAMEA6407434

General Information

Publications
Projects
* Is the cell line readily obtainable for third parties?
Yes

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases

External Databases (Donor)

BioSamples SAMEA6407438

Ethics#

Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number
How may genetic information associated with the cell line be accessed?

hESC Derivation#

Date of derivation 2002-04-29
Embryo stage Blastula with ICM and Trophoblast
PGD Embryo?
No
ZP removal technique Enzymatic
Cell isolation Immunosurgery
Cell seeding Isolated ICM

Culture Conditions#

Feeder cells mouse embryonic fibroblast cell
Cellfinder Ont Id: EFO_0004040
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: DMEM/F12 with Glutamax
Main protein source: Anti-Human-Serum (Sigma)
Serum concentration: %
Supplements
Fetal Calf Serum %
hrbFGF %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
SSEA-1
No
telomerase reverse transcriptase
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY

Other Genotyping (Cell Line)