SA121
CEBe012-A
General#
Donor Information#
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | |
Ethics#
| Alternatives to consent are available? | Yes |
| Alternatives to consent |
hESC Derivation#
| Date of derivation | 2002-04-29 |
| Embryo stage | Blastula with ICM and Trophoblast |
| PGD Embryo? |
No |
| ZP removal technique | Enzymatic |
| Cell isolation | Immunosurgery |
| Cell seeding | Isolated ICM |
Culture Conditions#
| Feeder cells |
mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040 |
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| O2 Concentration | 20 % | |||||||||
| CO2 Concentration | 5 % | |||||||||
| Medium |
Other medium:
Base medium: DMEM/F12 with Glutamax
Main protein source: Anti-Human-Serum (Sigma) Supplements
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Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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| SSEA-1 |
No |
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| telomerase reverse transcriptase |
Yes |
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Differentiation Potency
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
Genotyping#
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XY
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Other Genotyping (Cell Line) |
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