General#

Cell Line

hPSCreg Name
KUMi003-A
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 1st November 2021
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Provider

Generator Korea University Medical School Hospital (KUM)
Owner Korea University Medical School Hospital (KUM)
Distributors
Derivation country South Korea

External Databases

BioSamples SAMEA10580157
CLO CLO_0103560

General Information

* Is the cell line readily obtainable for third parties?
No

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 55-59
Ethnicity Korean

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
acute promyelocytic leukemia
Synonyms
  • AML with t(15;17)(q22;q12)
  • APML - acute promyelocytic leukemia
  • acute myeloid leukemia with t(15;17)(q22;q12);(PML/RARalpha) and variants
  • AML with t(15;17)(q22;q12);(PML/RARalpha) and variants
  • acute promyelocytic leukemia with PML-rara
  • AML M3
  • acute myeloblastic leukemia 3
  • acute promyelocytic leukemia with t(15;17)(q22;q12); PML/rara
  • acute promyelocytic leukemia with t(15;17)(q22;q12); PML-rara
  • APML
  • acute promyelocytic leukemia
  • promyelocytic leukemia
  • FAB M3
  • acute myeloblastic leukemia type 3
  • acute myeloid leukemia M3
  • Acute Promyelocytic Leukemia with t(15;17)(q22;q12); PML-RARA
  • Promyelocytic Leukemia
  • APML - Acute promyelocytic leukemia
  • APL
show more synonyms
Disease associated phenotypes no phenotypes
Family history N/A
Is the medical history available upon request? N/A
Is clinical information available? No

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA10580159

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Alternatives to consent are available? No
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? Yes
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? Yes
Does consent expressly prevent development of commercial products? No
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? No
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? Yes
Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? No
Does consent expressly permit collection of genetic information? Yes
Does consent expressly permit storage of genetic information? Yes
Does consent prevent dissemination of genetic information? Yes
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? Yes
Has the donor consented to receive information discovered during use of donated embryo/tissue that has significant health implications for the donor? Yes
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? Yes
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? Yes
Does consent permit access to medical records of the donor? Yes
Please describe how access is provided: Access anonymized medical record
Does consent permit access to any other source of information about the clinical treatment or health of the donor? Yes
Contact data, institution, or website: Access anonymized medical record
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Korea University Anam hospital IRB
Approval number K2020-0261-002
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Korea University Anam hospital IRB
Approval number K2020-0261-002
Do you have obligations to third parties in regard to the use of the cell line? No
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? Commercial kit used (Sendai Virus)
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation#

General

Source cell line name M3_CD34+iPSCs
Derived from same source line (potentially other lot and donor, see below):
Source cell type
Autologous CD34-positive Hematopoietic Progenitor Cells
A population of autologous CD34-positive hematopoietic progenitor cells (HPCs) that can be used for autotransplantation. CD34+ HPCs are isolated from human blood stem cells upon apheresis. Upon transplantation with the CD34+ HPCs, these cells can differentiate into a variety of cell types including fibroblasts, osteoblasts, chondrocytes, myocytes, adipocytes, and endothelial cells.
Synonyms
  • Autologous CD34-positive HPCs
  • Autologous CD34-positive Hematopoietic Progenitor Cells
Source cell origin
Bone Marrow
The tissue occupying the spaces of bone. It consists of blood vessel sinuses and a network of hematopoietic cells which give rise to the red cells, white cells, and megakaryocytes.
Synonyms
  • BONE MARROW
  • Bone Marrow
  • Reticuloendothelial System, Bone Marrow
  • bone marrow
show more synonyms
Source cell type (free text) Acute promyelocytic leukemia
Age of donor (at collection) 55-59
Collected in 2021
Passage number reprogrammed 3

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
Yes
Methods used
RT-PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Type of used vector free reprogramming factor(s)
mRNA

Other

Derived under xeno-free conditions
Yes
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Mechanically
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60
Yes
NANOG
Yes
SSEA-4
Yes
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
Morphology
Ectoderm
Ont Id: UBERON_0000924
Morphology

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Karyotype
Passage number: 5
Karyotyping method: G-Banding

Other Genotyping (Cell Line)