KUMi001-A_CML(Ph+)

General#

Cell Line

hPSCreg Name
KUMi001-A
Alternative name(s)
KUMi001-A_CML(Ph+)
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 19th July 2021
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Provider

Generator Korea University Medical School Hospital (KUM)
Owner Korea University Medical School Hospital (KUM)
Distributors
Derivation country South Korea

External Databases

BioSamples SAMEA9147487
CLO CLO_0103402

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information#

General Donor Information

Sex male
Ethnicity Korean

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Philadelphia-Positive Myelogenous Leukemia (primary disease)
The donor is a carrier of a disease-associated mutation and affected.
Stage
Chronic stage
Synonyms
  • Ph1-Positive Myeloid Leukemia
  • Ph1-Positive Myelogenous Leukemia
  • Philadelphia-Positive Myelocytic Leukemia
  • Philadelphia-Positive Myelogenous Leukemia
  • Philadelphia-Positive Myeloid Leukemia
  • Ph1-Positive Myelocytic Leukemia
  • Philadelphia-Positive Granulocytic Leukemia
  • Ph1-Positive Granulocytic Leukemia
show more synonyms
Family history NA
Is the medical history available upon request? NA
Is clinical information available? Yes

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46,XY,t(9;22)(q34;q11.2)
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA9147488

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Alternatives to consent are available? No
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? Yes
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? Yes
Does consent expressly prevent development of commercial products? No
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? No
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? Yes
Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? Yes
Does consent expressly permit collection of genetic information? Yes
Does consent expressly permit storage of genetic information? Yes
Does consent prevent dissemination of genetic information? Yes
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? Yes
Has the donor consented to receive information discovered during use of donated embryo/tissue that has significant health implications for the donor? Yes
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? Yes
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? Yes
Does consent permit access to medical records of the donor? Yes
Please describe how access is provided: access anonymized medical record
Does consent permit access to any other source of information about the clinical treatment or health of the donor? Yes
Contact data, institution, or website: access anonymized medical record
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? No
Do you have obligations to third parties in regard to the use of the cell line? No
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? commercial kits used (Sendai Virus)
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation#

General

Source cell line name MM_CD34+iPSCs
Derived from same source line (potentially other lot and donor, see below):
Source cell type
CD34 Positive
An indication that CD34 expression has been detected in a sample.
Synonyms
  • HPCA1 Positive
  • CD34 Antigen Positive
  • CD34 Positive
  • Hematopoietic Progenitor Cell Antigen CD34 Positive
  • CD34 Molecule Positive
show more synonyms
Source cell origin
Bone Marrow Mononuclear Cell
Any cell with a single nucleus found in the bone marrow, for example lymphocytes, monocytes, mesenchymal stem cells and hematopoietic progenitor cells.
Synonyms
  • BONE MARROW MONONUCLEAR CELL
  • Bone Marrow Mononuclear Cell
  • BMMC

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Mechanically
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60
Yes
POU5F1 (OCT-4)
Yes
Score:
Marker Present Absent
mCpG
OCT4
Morphology pictures
FACS_OCT4
Method documentation
Differentiation Potency

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY,t(9;22)(q34;q11.2)
Karyotyping method: G-Banding

Other Genotyping (Cell Line)