MM_CD34+iPSCs

General#

Cell Line

hPSCreg Name KUMCi002-A
Alternative name(s)
MM_CD34+iPSCs
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 4th September 2020
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Provider

Generator KOREA UNIVERSTY COLLEGE OF MEDICINE (KUMC)
Derivation country South Korea

External Databases

BioSamples SAMEA7462430

General Information

* Is the cell line readily obtainable for third parties?
No

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 45-49
Ethnicity Korean

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
multiple myeloma
Synonyms
  • Plasma-Cell Myelomas
  • Plasmacytic myeloma
  • Multiple myeloma, no ICD-O subtype
  • Myelomas, Plasma-Cell
  • MULT MYE W/O ACHV RMSON
  • Myeloma, NOS
  • Myeloma, Plasma Cell
  • Cell Myelomas, Plasma
  • Myeloma
  • Multiple myeloma (disorder)
  • Multiple myeloma without mention of having achieved remission
  • Kahler's disease
  • myeloma, plasma cell, malignant
  • Plasma Cell Myelomas
  • Myeloma, Multiple
  • myeloma
  • Myeloma Multiple
  • Myelomas, Plasma Cell
  • Myeloma-Multiples
  • Myeloma-Multiple
  • medullary plasmacytoma
  • Cell Myeloma, Plasma
  • Multiple myeloma without mention of remission
  • Disease, Kahler
  • multiple myeloma
  • Multiple myeloma, morphology (morphologic abnormality)
  • Multiple Myelomas
  • Myeloma, Plasma-Cell
  • myelomatosis
  • Plasma Cell Myeloma
  • myeloid neoplasm of plasma cell
  • [M]Plasma cell myeloma
  • Myelomas, Multiple
  • Myelomatoses
  • plasma cell myeloma
  • Myelomatosis
  • multiple myeloma/plasma cell myeloma
  • Multiple myeloma (clinical)
  • MULT MYELM W/O REMISSION
  • Kahler disease
  • Plasma-Cell Myeloma
  • plasma cell myeloid neoplasm
  • Multiple myeloma, no ICD-O subtype (morphologic abnormality)
show more synonyms
Disease associated phenotypes no phenotypes

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46,XX
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA7462431

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? Yes
If you do not hold the SIGNED Donor Consent Form, have you obtained a copy of the unsigned Donor Consent Form from the holder? No
Alternatives to consent are available? No
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? Yes
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent expressly prevent development of commercial products? Yes
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? Yes
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? Yes
Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes

Does consent permit research by

an academic institution? Yes
a public organisation? No
a non-profit company? Yes
a for-profit corporation? No
Does consent expressly permit collection of genetic information? Yes
Does consent expressly permit storage of genetic information? Yes
Does consent prevent dissemination of genetic information? Yes
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? Yes
Has the donor consented to receive information discovered during use of donated embryo/tissue that has significant health implications for the donor? Yes
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? Yes
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? Yes
Does consent permit access to medical records of the donor? No
Does consent permit access to any other source of information about the clinical treatment or health of the donor? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? No
Do you have obligations to third parties in regard to the use of the cell line? No
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation#

General

Source cell line name MM_CD34+iPSCs
Source cell type
CD34 Positive
An indication that CD34 expression has been detected in a sample.
Synonyms
  • HPCA1 Positive
  • CD34 Antigen Positive
  • CD34 Positive
  • Hematopoietic Progenitor Cell Antigen CD34 Positive
  • CD34 Molecule Positive
show more synonyms
Source cell origin
Bone Marrow Mononuclear Cell
Any cell with a single nucleus found in the bone marrow, for example lymphocytes, monocytes, mesenchymal stem cells and hematopoietic progenitor cells.
Synonyms
  • BONE MARROW MONONUCLEAR CELL
  • Bone Marrow Mononuclear Cell
  • BMMC
Age of donor (at collection) 45-49
Collected in 2020
Passage number reprogrammed 7

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
Yes
Methods used
PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones Colonies with morphology characteristic of iPSCs were confirmed using positive TRA-1-60 live staining and manually picked.
Derived under xeno-free conditions
Yes
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Dispase
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60
Yes
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
NANOG
Yes
SSEA-4
Yes
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
AFP – alpha fetoprotein
Yes
Morphology
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
Desmin
Yes
Morphology
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
TuJ1 (class III beta-tubulin)
Yes
Morphology

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX
Passage number: 7
Karyotyping method: G-Banding

Other Genotyping (Cell Line)