Generation of a homozygous CRISPR/Cas9-mediated knockout human iPSC line for PTCH1 gene
PTCH1 is the receptor protein of Hedgehog signaling pathway, and Hedgehog pathway plays a vital role in mammalian embryonic development. However, the specific biological role of PTCH1 is incompletely understood for embryonic development. Here, we used a CRISPR/Cas9 genome editing approach to generate a homozygous PTCH1 knock-out iPSC line (SCMCi001-A-1) from a healthy donor, which will be a valuable in vitro model to study the pathogenic mechanism of PTCH1 dysfunction in congenital disease. Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.
|Authors||Zu B, Zhang X, You G, Fu Q|
|Journal||Stem cell research|
|Publication Date||2021 Oct;56:102517|