Generation of the UAE's first Emirati induced pluripotent stem cell line KUSTi001-A from peripheral blood derived CD34+ hematopoietic cells
Summary
Here we report the generation of the first Emirati iPSC line in the United Arab Emirates and name it KUSTi001-A. CD34+ hematopoietic cells purified from peripheral blood of a 27-year-old healthy female donor were reprogrammed using Sendai vectors. Twenty days post-reprogramming colonies were manually picked, and expanded clones were verified for transgene clearance by RT-PCR. Pluripotency was validated by pluripotency genes and differentiation into all three germ layers. Finally, chromosome stability was confirmed by testing 8 common abnormality loci. KUSTi001-A, alternatively called UAE001, is an Emirati hiPSC line that holds great potential for UAE specific regenerative medicine, disease modelling and drug screening. Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Azzam SK, Alsafar H, Sajini AA |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2022 Aug;63:102853 |
| PubMed | 35816920 |
| DOI | 10.1016/j.scr.2022.102853 |