KAUSTi004-B

HM-iPSC#B

General#

Cell Line

hPSCreg Name KAUSTi004-B
Alternative name(s)
HM-iPSC#B
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 27th August 2020
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Provider

Generator King Abdullah University of Science and Technology (KAUST)
Owner King Abdullah University of Science and Technology (KAUST)
Distributors
Derivation country Saudi Arabia

External Databases

BioSamples SAMEA7381961
Cellosaurus CVCL_A9PX
CLO CLO_0102954

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 40-44
Ethnicity Caucasian-Sardinian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
initial karyotype analysis reveals subject is 46,XX with random chromosome losses seen in 12% of cells, random chromosome gains seen in 2% of cells, and random chromosome aberrations seen in 2% of cells
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor
All cell lines of this donor's relatives
Has son:

External Databases (Donor)

BioSamples SAMEA7381960

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: NIGMS Human Genetic Cell Repository
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? No
If you do not hold the SIGNED Donor Consent Form, do you know who does? No
If you do not hold the SIGNED Donor Consent Form, have you obtained a copy of the unsigned Donor Consent Form from the holder? No
Alternatives to consent are available? Yes
Alternatives to consent https://www.coriell.org/1/NIGMS
Alternative consent approval number
Has the donor agreed to be re-contacted? Unknown
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? Yes
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Fibroblasts GM00321 were obtained from the NIGMS Human Genetic Cell Repository at the Coriell Institute for Medical Research, USA.
Approval number
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? KAUST Institutional Biosafety and Bioethics Committee
Approval number 17IBEC14
Is there an MTA available for the cell line? Yes
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? Stemgent

hIPSC Derivation#

General

Source cell line name GM00321
Derived from same source line (potentially other lot and donor, see below):
Source cell type
Fibroblasts
Age of donor (at collection) 40-44
Source cell line vendor Coriell
Passage number reprogrammed 8

Reprogramming method

Vector type None

Vector free reprogramming

Type of used vector free reprogramming factor(s)
mRNA, Small molecules, miRNA
mRNA
Small molecules

Other

Selection criteria for clones Clones were stained using the live imaging antibody TRA1-60 and among the positive colonies the ones with best stem cell morphology were selected for picking and expansion.
Derived under xeno-free conditions
Yes
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
TrypLE
O2 Concentration 5 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
NANOG
Yes
SOX2
Yes
SSEA-1
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Marker Expressed
Cytokeratin
Yes
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Marker Expressed
Desmin
Yes
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
Marker Expressed
S100
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX
Passage number: 13
Karyotyping method: G-Banding

Other Genotyping (Cell Line)