Generation of an iPSC cell line (USFi003-A) from a patient with dilated cardiomyopathy carrying a heterozygous mutation in LMNA (p.R541C)
Summary
Mutations in the gene that encodes the nuclear envelope proteins lamin A/C (LMNA) are considered to be a prominent cause of Dilated cardiomyopathy (DCM), a leading cause of heart failure and a prevalent indication for heart transplant. Here we described the generation of induced pluripotent stem cells (iPSCs) from a 53-year-old female with DCM plus progressive conduction disease who carry a heterozygous mutation in LMNA (c.1621C > T, p.R541C). PBMCs isolated from the patient were reprogrammed with Yamanaka factors KOS, KLF4, and c-MYC by the non-integrating sendai virus vector system. The obtained iPSC lines demonstrated normal karyotype and pluripotent identity. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Yang J, Burgos Angulo M, Argenziano MA, Bertalovitz A, Najari Beidokhti M, McDonald TV |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2021 Jul;54:102396 |
| PubMed | 34029931 |
| DOI | 10.1016/j.scr.2021.102396 |