Generation of an iPSC cell line (USFi003-A) from a patient with dilated cardiomyopathy carrying a heterozygous mutation in LMNA (p.R541C)


Mutations in the gene that encodes the nuclear envelope proteins lamin A/C (LMNA) are considered to be a prominent cause of Dilated cardiomyopathy (DCM), a leading cause of heart failure and a prevalent indication for heart transplant. Here we described the generation of induced pluripotent stem cells (iPSCs) from a 53-year-old female with DCM plus progressive conduction disease who carry a heterozygous mutation in LMNA (c.1621C > T, p.R541C). PBMCs isolated from the patient were reprogrammed with Yamanaka factors KOS, KLF4, and c-MYC by the non-integrating sendai virus vector system. The obtained iPSC lines demonstrated normal karyotype and pluripotent identity. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Yang J, Burgos Angulo M, Argenziano MA, Bertalovitz A, Najari Beidokhti M, McDonald TV
Journal Stem cell research
Publication Date 2021 Jul;54:102396
PubMed 34029931
DOI 10.1016/j.scr.2021.102396

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