Human induced pluripotent stem cell (iPSC) line (HEBHMUi014-A) derived from a patient with Alzheimer's disease
Summary
The ε4 allele of the lipoprotein E gene (APOE4) is the strongest genetic risk factor associated with sporadic Alzheimer's disease (sAD). While the neuronal cell type-specific function of APOE4 in connection with AD pathology remains understudied. Therefore, we generated an induced pluripotent stem cells (iPSCs) line from a 77-year-old female donor with ApoE4 genetic background. We reprogrammed peripheral blood mononuclear cells (PBMCs) with non-integrative Sendai viral vectors containing reprogramming factors. Established iPSCs showed the capability of pluripotency, three-germ differentiation in vitro with normal karyotype. Hence, the generated iPSC could be a powerful tool to conduct further studies of AD mechanisms. Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Zhang M, Yu M, Guo R, Zhang Y, Zhang X, Sun J, Zhang J, Wang X, Ma J |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2023 Jun;69:103116 |
| PubMed | 37178573 |
| DOI | 10.1016/j.scr.2023.103116 |