SGS_I871I
OSRi010-A-1
General
Cell Line |
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| hPSCreg name | OSRi010-A-1 |
| Cite as: | OSRi010-A-1 (RRID:CVCL_A0YB) |
| Alternative name(s) |
SGS_I871I
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
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| Last update | 17th August 2021 |
| Notes | This line represents the correction by CRISPR of the Schinzel-Giedion mutation I871T of the line SGS_I871T (OSRi010-A) |
| User feedback | |
Provider |
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| Generator |
Ospedale San Raffaele (OSR)
Contact:
Ospedale San Raffaele (OSR) |
| Derivation country | Italy |
External Databases |
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| BioSamples | SAMEA9687262 |
| Cellosaurus | CVCL_A0YB |
| Wikidata | Q108821082 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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| Disease associated phenotypes |
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Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Unknown
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
Yes
Exome sequencing
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External Databases (Donor) |
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| BioSamples | SAMEA9687261 |
Ethics
Also have a look at the ethics information for the parental line
OSRi010-A
.
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
OSRi010-A.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
| Is reprogramming vector detectable? |
Unknown |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Medium |
mTeSR™ 1
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| SOX2 |
Yes |
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| TRA 1-60 |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XY
Passage number: P30
Karyotyping method:
Q-banding
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Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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