Generation of an integration-free iPSC line, ICCSICi006-A, derived from a male Alzheimer's disease patient carrying the PSEN1-G206D mutation


The familial form of Alzheimer's disease (FAD), which is caused by mutations in PRESENILIN 1 (PSEN1) and amyloid precursor protein (APP) genes, represents less than 5% of all AD cases and has an early-onset. We report the generation and characterization of an iPSC line derived from a FAD patient carrying the PSEN1-G206D mutation. The iPSC line maintained the original genotype, a normal karyotype, was free from Sendai viral vectors and reprogramming factors (OCT4, SOX2, KLF4 and c-MYC), presented a typical morphology, expressed endogenous pluripotency markers, and could be differentiated into ectodermal, mesodermal and endodermal cells, confirming its pluripotency. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Díaz-Guerra E, Oria-Muriel MA, Moreno-Jiménez EP, de Rojasb I, Rodríguez C, Rodríguez-Traver E, Orera M, Hernándezb I, Ruizb A, Vicario C
Journal Stem cell research
Publication Date 2019 Oct;40:101574
PubMed 31627126
DOI 10.1016/j.scr.2019.101574

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