Establishment of a CPAMD8-GFP reporter human embryonic stem cell line, IBBDe001-B, using CRISPR/Cas9 editing
Summary
The human CPAMD8 gene encodes proteins in the A2M/C3 (alpha-2-macroglobulin/complement 3) family, predominantly expressed in the distal tips of the retinal neuroepithelium that form the iris and ciliary body. Mutations in CPAMD8 have been linked to anterior segment dysplasia and congenital glaucoma. Using CRISPR/Cas9 editing, we inserted a 3*EAAAK-EGFP fluorescent tag into the CPAMD8 gene, enabling real-time observation of its expression and providing insights into its biological functions. The resulting gene-edited cell line retained normal stem cell morphology and karyotype, expressed essential pluripotency markers, and exhibited differentiation potential. Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Zhang S, Li J, Pan W, Ren Q, Zhou Y, Chen M, Qu J, Li S |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Dec;81:103615 |
| PubMed | 39603092 |
| DOI | 10.1016/j.scr.2024.103615 |