HLA-I/II-null SFiPSCs
MUSIi001-A-2
General
Donor Information
General Donor Information |
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Sex | female |
Ethnicity | Thai |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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Family history | N/A |
Is the medical history available upon request? | N/A |
Is clinical information available? | N/A |
Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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Other cell lines of this donor | |
External Databases (Donor) |
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BioSamples | SAMEA104627073 |
Ethics
Also have a look at the ethics information for the parental line
MUSIi001-A
.
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
MUSIi001-A.
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Reprogramming method |
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Vector type | Non-integrating |
Vector | Sendai virus |
Genes | |
Is reprogramming vector detectable? |
No |
Methods used |
RT-PCR
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Vector free reprogramming |
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Type of used vector free reprogramming factor(s) |
None
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Other |
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Selection criteria for clones | The iPSC clone that do not express HLA-I/II was selected. |
Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
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O2 Concentration | 20 % |
CO2 Concentration | 5 % |
Medium |
Essential 8™
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Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
NANOG |
Yes |
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SSEA-4 |
Yes |
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TRA 1-60 |
Yes |
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TRA 1-81 |
Yes |
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OCT-4 |
Yes |
Differentiation Potency
Microbiology / Virus Screening |
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Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
Genetic Modification
Genetic modifications not related to a disease |
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