Phenotypic and transcriptomic profiling of induced pluripotent stem cell (iPSC)-derived NK cells and their cytotoxicity against cancers
Summary
This study highlights the potential of iPSCs as an effective alternative cell source for generating NK cells. Using a two-step in vitro monolayer feeder-free system, we successfully generated iNK cells that not only expressed key NK cell markers and their receptors but also displayed a transcriptomic profile closely resembling PB-NK cells. Furthermore, iNK cells exhibited cytotoxicity against CCA and BCA cell lines comparable to that of PB-NK cells. This approach could pave the way for off-the-shelf NK cell products, potentially enhancing the effectiveness of adoptive NK cell therapy. © 2024. The Author(s).
| Authors | Thongsin N, Suwanpitak S, Augsornworawat P, Srisantitham J, Saiprayong K, Jenjaroenpun P, Wattanapanitch M |
|---|---|
| Journal | Stem cell research & therapy |
| Publication Date | 2024 Nov 13;15(1):418 |
| PubMed | 39533434 |
| PubMed Central | PMC11559060 |
| DOI | 10.1186/s13287-024-04029-z |