cFA404-KiPS4F-3

General#

Cell Line

hPSCreg Name ESi005-B
Alternative name(s)
cFA404-KiPS4F-3
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 25th May 2018
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Provider

Generator Spanish Stem Cell Bank (ES)
Owner Centre of Regenerative Medicine in Barcelona - Banc de Linies Cellulars (CRMB)
Distributors
Derivation country Spain

External Databases

Cellosaurus CVCL_C193

General Information

Publications
Is the cell line available in principle?
Yes

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Fanconi anemia
The donor is a carrier of a disease-associated mutation.
Synonyms
  • Fanconi pancytopenia
Genetic variants
FANCD2 (target)
3p25.3

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA3303136

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
keratinocyte
An epidermal cell which synthesizes keratin and undergoes a characteristic change as it moves upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell.
Synonyms
  • malpighian cell
  • keratinized cell of epidermis
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Source cell type (free text) The gene FANCD2 (3p25.3) was knocked out in the keratinocytes before iPS reprogramming as a genetic correction of Fanconi anemia.

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Genes
Is the used vector excisable?
No
Absence of reprogramming vector(s)?
Unkown
Reprogramming vectors silenced?

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Gelatin
Feeder cells Human Foreskin Fibroblast
Cellfinder Ont Id: CELDA_000001419
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-Glutamine
bFGF

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
SOX2
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Marker Expressed
AFP
Yes
FOXA2
Yes
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Marker Expressed
alpha-actinina
Yes
ASA
Yes
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation
Marker Expressed
Tuj1
Unkown
TH
Unkown
GFP
Unkown

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)