Generation of the human erythroblast-derived iPSC line UBTi001-A


We performed reprogramming of human erythroblasts derived from CD34+ hematopoietic stem / progenitor cells of a healthy donor. CD34+ cells were differentiated in-vitro into a pure population of CD36+ erythroblasts and nucleofected with four episomal plasmids expressing SOX2, OCT3/4, KLF4, LIN28, L-MYC and TP53-shRNA. The established iPSC line showed normal karyotype. Pluripotency was confirmed by expression of pluripotency markers and in-vitro differentiation into tissues of all three germ layers. The UBTi001-A iPSC line might provide an attractive source for developmental research on human hematopoiesis and erythropoiesis. Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Avdili A, Rohrhofer L, Auer M, Bernecker C, Schlenke P, Dorn I
Journal Stem cell research
Publication Date 2022 Sep 7;64:102910
PubMed 36103773
DOI 10.1016/j.scr.2022.102910

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