AD-APP hiPSC

General

Cell Line

hPSCreg name IRMBi002-A
Cite as:
IRMBi002-A (RRID:CVCL_VD87)
Alternative name(s)
AD-APP hiPSC
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
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APOE, APOE
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Alzheimer disease
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APOE, APOE
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STBCi006-A-4
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APOE, APOE
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UMi042-A-1
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UKBi011-A-1
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APOE, APOE
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Alzheimer disease
UKBi011-A-2
(ApoE 2/2)
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APOE, APOE
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Alzheimer disease
UKBi011-A-3
(ApoE 3/3)
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APOE, APOE
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Alzheimer disease
UKBi011-A-4
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APOE, APOE
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Alzheimer disease
ZZUi009-A
(ZZU-iPS-AD-PS1-001)
Donor diseases:
obsolete_Alzheimer's disease
STBCi011-A
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STBCi011-B
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STBCi012-A
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STBCi013-A
(SFC807-03-01)
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(SFC807-03-04)
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PSEN1
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STBCi014-A
(SFC808-03-03)
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PSEN1
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(SFC808-03-04)
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Alzheimer disease
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(SFC808-03-06)
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(SFC039-03-01)
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(SFC039-03-02)
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PSEN1
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STBCi009-B
(SFC801-03-02)
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PSEN1
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STBCi009-C
(SFC801-03-03)
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PSEN1
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(SFC802-03-06)
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PSEN1
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Alzheimer disease
STBCi015-A
(SFC809-03-01)
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APP
Donor diseases:
Alzheimer disease
STBCi015-B
(SFC809-03-03)
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APP
Donor diseases:
Alzheimer disease
STBCi015-C
(SFC809-03-04)
Donor's gene variants:
APP
Donor diseases:
Alzheimer disease
STBCi047-A
(SFC043-03-01)
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Alzheimer disease
STBCi051-A
(SFC056-04-03)
Donor diseases:
Alzheimer disease
STBCi047-B
(SFC043-03-13)
Donor diseases:
Alzheimer disease
STBCi051-B
(SFC056-04-01)
Donor diseases:
Alzheimer disease
STBCi051-C
(SFC056-04-02)
Donor diseases:
Alzheimer disease
STBCi065-A
(SFC853-03-03)
Donor diseases:
Alzheimer disease
STBCi071-A
(SFC057-07-03)
Donor diseases:
Alzheimer disease
Last update 5th October 2022
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Provider

Generator Institute for Regenerative Medecine and Biotherapy (IRMB)
Owner Institute for Regenerative Medecine and Biotherapy (IRMB)
Distributors
Derivation country France

External Databases

Cellosaurus CVCL_VD87
BioSamples SAMEA4984140
Wikidata Q54898251

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Disease, Alzheimer
  • Dementia in Alzheimer's disease, unspecified (disorder)
  • Presenile Alzheimer Dementia
  • ALZHEIMERS DIS
  • Alzheimers
  • DAT - Dementia Alzheimer's type
  • Dementia in Alzheimer's disease
  • Alzheimer's disease, NOS
  • Alzheimer Dementia, Presenile
  • Dementia, Alzheimer Type
  • Alzheimer's
  • Dementia, Presenile
  • [X]Dementia in Alzheimer's disease (disorder)
  • ALZHEIMER DIS
  • AD
  • [X]Dementia in Alzheimer's disease
  • AD - Alzheimer's disease
  • Disease, Alzheimer's
  • Dementia, Presenile Alzheimer
  • Alzheimer Type Dementia
  • Dementia in Alzheimer's disease (disorder)
  • Alzheimer's disease (disorder)
  • Dementia of the Alzheimer's type
  • sporadic Alzheimer's disease
  • Alzheimers disease
  • Alzheimer Dementia
  • Alzheimer's Dementia
  • Alzheimer Disease
  • Alzheimers Dementia
show more synonyms

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA104618766

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information Dr David Wallon, Pr Alexis Brice, INSERM Rouen
Alternatives to consent are available? No
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? Unknown
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? Yes
Does consent expressly prevent development of commercial products? No
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? No
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? Yes
Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? No
Does consent expressly permit collection of genetic information? Yes
Does consent expressly permit storage of genetic information? Yes
Does consent prevent dissemination of genetic information? No
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? No
Has the donor consented to receive information discovered during use of donated embryo/tissue that has significant health implications for the donor? Yes
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? Yes
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? Yes
Does consent permit access to medical records of the donor? Yes
Please describe how access is provided: controlled access anonymised
Does consent permit access to any other source of information about the clinical treatment or health of the donor? Yes
Contact data, institution, or website: INSERM CHU ROuen
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? CPP Nord-Ouest 1
Approval number 2008/067/HP 110-05
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Direction Générale de la recherche et de l'innovation
Approval number IE-2013-675(4)/DC-2015-2363
Do you have obligations to third parties in regard to the use of the cell line? Yes
Please describe: INSERM has the intellectual property
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? ThermoFisher
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Passage number reprogrammed 3

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Other

Selection criteria for clones Morphology and in vivo detection of SSEA4 Thermofisher Kit
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Gelatin
Feeder cells MEF
Passage method Mechanically
O2 Concentration 5 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: KODMEM/F12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
NEAA 1 %
b-mercaptoethanol 0,1 mM
L-Glutamine 1 mM
Penicillin 100 U/ml
Streptomycin 100 µg/ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
NANOG
Yes
SOX2
Yes
SSEA-4
Yes
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
AFP
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
a-SMA
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
BIII-tubulin
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Passage number: 26
Karyotyping method: G-Banding

Other Genotyping (Cell Line)