ES-10
ESe025-A
General
Cell Line |
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hPSCreg name | ESe025-A |
Cite as: | ESe025-A (RRID:CVCL_C767) |
Alternative name(s) |
ES-10
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Cell line type | Human embryonic stem cell (hESC) |
Similar lines | No similar lines found. |
Last update | 28th July 2017 |
User feedback | |
Provider |
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Generator | Spanish Stem Cell Bank (ES) |
Derivation country | Spain |
External Databases |
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BioSamples | SAMEA7780168 |
Cellosaurus | CVCL_C767 |
Wikidata | Q54832749 |
General Information |
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Publications | |
Projects | |
* Is the cell line readily obtainable for third parties? |
Yes |
Donor Information
General Donor Information |
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Sex | female |
Phenotype and Disease related information (Donor) |
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Diseases | |
External Databases (Donor) |
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BioSamples | SAMEA7779418 |
Ethics
Was the embryo established purely for research purposes? | Yes |
Have both parents consented to the use of the embryo for ESC derivation? | Yes |
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
Was the consent voluntarily given? | Yes |
Alternatives to consent are available? | Yes |
Alternatives to consent | |
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
Does consent expressly prevent the derivation of pluripotent stem cells? | No |
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | Yes |
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
Name of accrediting authority involved? | Ethics Board of the Center of Regenerative Medicine in Barcelona |
Approval number |
hESC Derivation
Date of derivation | 2009-04-10 |
Embryo stage | Blastula with ICM and Trophoblast |
Supernumerary embryos from IVF treatment? |
Yes
|
PGD Embryo? |
No |
Expansion status |
4 |
ICM morphology |
A |
Trophectoderm morphology |
a |
ZP removal technique | Enzymatic |
Cell isolation | Laser |
Cell seeding | Isolated ICM |
Culture Conditions
Feeder cells |
Human Foreskin Fibroblast Cellfinder Ont Id: CELDA_000001419 |
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O2 Concentration | 20 % | |||||||||
CO2 Concentration | 5 % | |||||||||
Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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Characterisation
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
Alkaline Phosphatase |
Yes |
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NANOG |
Yes |
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POU5F1 (OCT-4) |
Yes |
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SSEA-3 |
Yes |
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SSEA-4 |
Yes |
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TRA 1-60 |
Yes |
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TRA 1-81 |
Yes |
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SOX2 |
Yes |
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Differentiation Potency
Microbiology / Virus Screening |
|
HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Genotyping
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
46XX
Passage number: 18
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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