RC11, RC-11

General#

Cell Line

hPSCreg Name RCe015-A
Alternative name(s)
RC11, RC-11
Cell line type Human embryonic stem cell (hESC)
Last update 28th November 2017
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Provider

Generator Roslin Cells (RC)
Owner Derivation of Clinical Grade hES Cells
Distributors
Derivation country United Kingdom

External Databases

Cellosaurus CVCL_C782

General Information

Publications
Projects
Is the cell line available in principle?
Yes

Donor Information#

General Donor Information

Phenotype and Disease related information (Donor)

Diseases

Ethics#

Alternatives to consent are available? Yes
Alternatives to consent
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? HFEA

hESC Derivation#

Embryo stage Cleavage (Mitosis)
ZP removal technique Mechanical
Cell isolation None
Cell seeding Isolated ICM
Derivation under GMP?
Yes

Culture Conditions#

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
SSEA-1
No

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative

Genotyping#

Karyotyping (Cell Line)

Other Genotyping (Cell Line)