MasterShef10, MShef10, MstrShef10

General

Cell Line

hPSCreg Name
UOSe014-A
Alternative name(s)
MasterShef10, MShef10, MstrShef10
Cell line type Human embryonic stem cell (hESC)
Last update 7th February 2022
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Provider

Generator University of Sheffield (UOS)
Owner Centre for Stem Cell Biology
Distributors

External Databases

BioSamples SAMEA104627967
Cellosaurus CVCL_BW82
CLO CLO_0101515
Wikidata Q54904041

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: allowed

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history No
Is the medical history available upon request? No
Is clinical information available? No

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA104627968

Ethics

Was the embryo established purely for research purposes? No
Have both parents consented to the use of the embryo for ESC derivation? Yes
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Contact information / weblink https://www.carefertility.com/our-clinics/ivf-clinic-northampton/
Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? National Research Ethics Service - North West London Rec-2
Approval number 05/Q0501/63

hESC Derivation

Date of derivation 2012-03-21
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No
Expansion status 3
Stephenson 2007
ICM morphology B
Stephenson 2007
Trophectoderm morphology b
Stephenson 2007
ZP removal technique Laser
Cell isolation Mechanical
Cell seeding Embryo
Derived under xeno-free conditions?
No
Derivation under GMP?
Yes
Available as clinical grade?
Yes

Culture Conditions

Surface coating Non coated
Feeder cells Human Foreskin Fibroblasts
Cellfinder Ont Id: CELDA_000001419
Passage method Mechanically
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: Nutristem
Main protein source: Human Serum Albumin
Serum concentration: 5 %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-1
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-81
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
alpha fetal protein
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
Desmin
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
Beta Tubulin III
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage:

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Passage number: P12
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Exome sequencing
Two TP53 Mutations detected (R248W and G245S)