Establishment of SIAISi021-A, an induced pluripotent stem cell (iPSC) line from a 71-year-old Chinese Han male with Alzheimer's Disease (AD) having two copies of APOE4/4 allele
Summary
A 71-year-old Han male from China contributed peripheral blood mononuclear cells (PBMCs). Non-integrative Sendai viral vectors containing reprogramming factors OCT4, KLF4, SOX2 and C-MYC were used to reprogram PBMCs. Pluripotency makers confirmed the pluripotency of transgene-free induced pluripotent stem cell (iPSC). The ability of iPSC to spontaneously differentiate three germ layers in vitro confirmed the pluripotency of iPSC. The iPSC line displayed a normal karyotype. The newly generated human iPSC SIAISi021-A can be used for studying further disease mechanisms of Alzheimer's Disease (AD). Copyright © 2022 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Ding Y, Chen H, Zhao J, Wang Y, Deng Y |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2022 Aug;63:102866 |
| PubMed | 35843023 |
| DOI | 10.1016/j.scr.2022.102866 |