MCRIi-TC160154-GC-cl5.30.4
The cell line is not validated yet.
MCRIi035-B-2
General
Cell Line |
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| hPSCreg name | MCRIi035-B-2 |
| Cite as: | MCRIi035-B-2 |
| Alternative name(s) |
MCRIi-TC160154-GC-cl5.30.4
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 20th January 2025 |
| User feedback | |
Provider |
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| Generator | Murdoch Children's Research Institute (MCRI) |
| Owner | Murdoch Children's Research Institute (MCRI) |
| Distributors | |
| Derivation country | Australia |
General Information |
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| Projects | |
| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | European |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
Ethics
Also have a look at the ethics information for the parental line
MCRIi035-B
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | Thermo Fisher Scientific |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
MCRIi035-B.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
Unknown |
Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
mRNA
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Other |
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| Selection criteria for clones | Manually. Morphology and growth then sequencing and characterisation |
| Derived under xeno-free conditions |
Yes |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
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| CO2 Concentration | 5 % |
| Medium |
TeSR™ E8™
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| NANOG |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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Morphology pictures
Differentiation Potency
In vitro directed differentiation
In vitro directed differentiation
Microbiology / Virus Screening |
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| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
Arr(X,1-22)x2
Passage number: <25
Karyotyping method:
Array CGH
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Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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