Generation of an erythroid progenitor-derived iPSC line, VRISGi002-A, from a healthy 27-year-old Vietnamese donor under a feeder-free system
Summary
We have established the footprint-free Vietnamese human induced pluripotent stem cell (hiPSC) line, VRISGi002-A, from CD71 + CD235a + erythroid progenitor cells (EPCs) of a 27-year-old healthy donor. The EPCs were enriched from isolated peripheral blood and reprogrammed using Sendai viruses which carried the reprogramming factors c-MYC, SOX2, KLF4, and OCT4 under a feeder-free culture system. The established VRISGi002-A cell line expressed typical pluripotency markers, displayed a normal karyotype, and demonstrated the potential to differentiate into the three germ layers. This hiPSC line could serve as a Vietnamese healthy control model for physiological processes and drug screening. Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Nhung Nguyen TH, Trinh Tran TT, Luong TH, Nguyen XH |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2022 Jul;62:102824 |
| PubMed | 35679757 |
| DOI | 10.1016/j.scr.2022.102824 |