Generation of two human ISG15 knockout iPSC clones using CRISPR/Cas9 editing


Interferon stimulated gene 15 (ISG15) is one of the most highly upregulated proteins in response to viral infection and is involved in numerous pathways with multiple mechanisms of actions. ISG15 deficiency has been reported to induce type I interferonopathy owing to defective negative regulation of IFN-I signalling as well as enhanced antiviral protection. Here, we have generated ISG15 knockout clones from human iPSCs, which provide useful cell resources to study mechanisms of ISG15 deficiency and gain more insight into the biological function of ISG15. Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Merkert S, Jaboreck MC, Engels L, Malik MNH, Göhring G, Pessler F, Martin U, Olmer R
Journal Stem cell research
Publication Date 2020 Dec 22;50:102135
PubMed 33383405
DOI 10.1016/j.scr.2020.102135

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