GENEAe011-A

SIVF12, GENEA012

The cell line is not validated yet.

General#

Cell Line

hPSCreg Name
GENEAe011-A
Alternative name(s)
SIVF12, GENEA012
Cell line type Human embryonic stem cell (hESC)
Last update 22nd May 2019
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Genea (GENEA)
Contact:
Biocells
Distributors
Derivation country Australia

External Databases

BioSamples SAMEA104012420
Cellosaurus CVCL_9021
CLO CLO_0100335
Wikidata Q54835574

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

External Databases (Donor)

BioSamples SAMEA104012419

Ethics#

Alternatives to consent are available? Yes
Alternatives to consent
Name of accrediting authority involved?
Approval number

hESC Derivation#

Embryo stage Blastula with ICM and Trophoblast
PGD Embryo?
No
ZP removal technique Enzymatic
Cell isolation Mechanical
Cell seeding Isolated ICM

Culture Conditions#

Passage method Mechanically
Medium Other medium:
Base medium: DMEM/F-12
Main protein source: Fetal bovine serum
Serum concentration: 20 %
Supplements
L-Glutamine
bFGF

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SSEA-1
No
SSEA-4
Yes
TRA 1-60
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Passage number: 27

Other Genotyping (Cell Line)