SIVF12, GENEA012
The cell line is not validated yet.
GENEAe011-A
General
Cell Line |
|
| hPSCreg name | GENEAe011-A |
| Cite as: | GENEAe011-A (RRID:CVCL_9021) |
| Alternative name(s) |
SIVF12, GENEA012
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 22nd May 2019 |
| User feedback | |
Provider |
|
| Generator |
Genea (GENEA)
Contact:
Biocells |
| Derivation country | Australia |
External Databases |
|
| BioSamples | SAMEA104012420 |
| Cellosaurus | CVCL_9021 |
| Wikidata | Q54835574 |
General Information |
|
| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
|
| Sex | female |
Phenotype and Disease related information (Donor) |
|
| Diseases | |
External Databases (Donor) |
|
| BioSamples | SAMEA104012419 |
Ethics
| Alternatives to consent are available? | Yes |
| Alternatives to consent | |
| Name of accrediting authority involved? | |
| Approval number |
hESC Derivation
| Embryo stage | Blastula with ICM and Trophoblast |
| PGD Embryo? |
No |
| ZP removal technique | Enzymatic |
| Cell isolation | Mechanical |
| Cell seeding | Isolated ICM |
Culture Conditions
| Passage method | Mechanically | |||||||||
| Medium |
Other medium:
Base medium: DMEM/F-12
Main protein source: Fetal bovine serum Serum concentration: 20 % Supplements
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
|
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| POU5F1 (OCT-4) |
Yes |
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| SSEA-1 |
No |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46XX
Passage number: 27
|
Other Genotyping (Cell Line) |
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