Generation of a homozygous ABCA7-knockout human iPSC line using the CRISPR/Cas9 system


ABCA7 encodes an ATP-binding cassette transporter, and its loss-of-function variants are associated with Alzheimer's disease. To investigate the role of ABCA7 deficiency in the pathogenesis of Alzheimer's disease, we generated a homozygous ABCA7-knockout induced pluripotent stem cell (iPSC) line using CRISPR/Cas9-mediated gene editing. This ABCA7-deficient iPSC line maintains a normal karyotype, expression of pluripotency markers, and trilineage differentiation capacity. Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Guan M, Chai Y, Yi R, Chen Y, Ip JPK, Ye T, Chen Y
Journal Stem cell research
Publication Date 2023 Feb;66:103000
PubMed 36521383
DOI 10.1016/j.scr.2022.103000

Research Projects

Cell Lines