Generation of a homozygous ZBTB7A knockout human induced pluripotent stem line by CRISPR/Cas9 editing
Summary
ZBTB7A plays important roles in several biological processes, including silencing of the fetal γ-globin genes, hematopoiesis, primed-to-naive transition, etc. Meanwhile, it is also associated with Oncogenic transformation and tumor progression. However, the mechanism of ZBTB7A function is not fully understood yet. Here, we generated a homozygous ZBTB7A knockout human induced pluripotent stem cell (iPSC) line, GZHMCi007-A by the CRISPR/Cas9-mediated homology-dependent DNA repair method. The iPSCs of ZBTB7A-/- established by us is a powerful tool for related research. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Chen D, Song B, Cheng Y, Zhu L, Lu D, Liu N, Yang Y, Sun X |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2021 Aug;55:102494 |
| PubMed | 34403853 |
| DOI | 10.1016/j.scr.2021.102494 |