Generation of a human induced pluripotent stem cell line (INEUi001-A) from an amyotrophic lateral sclerosis/frontotemporal dementia patient with a C9ORF72 G4C2 genotype of <2 (GGGGCCG) and 10 repeats


Human induced pluripotent stem cell (hiPSC) line INEUi001-A was reprogrammed from peripheral blood mononuclear cells (PBMC) using the lentiviral-hSTEMCCA-loxP vector. PBMCs were obtained from a 75- year-old female ALS/FTD disease patient carrying a heterozygous deletion within the C9ORF72 hexanucleotide repeat region resulting in a GGGGCCG sequence (∼1.16 repeats). C9ORF72 genotype was maintained and stemness and pluripotency confirmed in INEUi001-A hiPSC line. Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Nievas M, Romorini L, Isaja L, Clas GS, Rodríguez-Varela S, Mucci S, Itzcovich T, de Ambrosi B, Scassa ME, Sevlever GE, Surace EI, Marazita MC
Journal Stem cell research
Publication Date 2023 Jun;69:103076
PubMed 36963214
DOI 10.1016/j.scr.2023.103076

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