Derivation of two induced pluripotent stem cell lines from a healthy control subject
Summary
Two human induced pluripotent stem cell lines, LEIi021-A and LEIi021-B, were derived from dermal fibroblasts from a healthy control subject from an Australian Aboriginal family with retinitis pigmentosa-11. Reprogramming was performed using episomal vectors expressing OCT4, SOX2, LIN28, L-MYC, KLF4 and mp53DD. Pluripotency markers were expressed in both LEIi021-A and LEIi021-B lines. The two cell lines displayed normal karyotypes and demonstrated the ability to differentiate into embryoid bodies with the three primary germ layers and retinal pigment epithelial cells. Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Zhang D, Huang D, Larcher LM, Zaw K, Chen SC, Jennings L, Lamey TM, Thompson JA, McLaren TL, Chen FK, McLenachan S |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2025 Feb;82:103621 |
| PubMed | 39642595 |
| DOI | 10.1016/j.scr.2024.103621 |