SYSUSCi004-A

IMO-hiPSCs

General

Cell Line

hPSCreg name SYSUSCi004-A
Cite as:
SYSUSCi004-A (RRID:CVCL_D0M3)
Alternative name(s)
IMO-hiPSCs
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 28th July 2023
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Provider

Generator Sun Yat-Sen University, center for stem cell biology and tissue engineering (SYSUSC)

External Databases

BioSamples SAMEA114184492
Cellosaurus CVCL_D0M3
Wikidata Q123033584

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • Infantile malignant osteopetrosis
Genetic variants
TCIRG1 (target)
NM_006019: c.242delC, c.1114C>T

External Databases (Donor)

BioSamples SAMEA114184493

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? the ethics committee of Zhongshan School of Medicine, Sun Yat-sen University
Approval number 2022-014
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • PBMC
Passage number reprogrammed 1

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
NANOG
Yes
SOX2
Yes
SSEA-4
Yes
TRA 1-60
Yes
Score:
Marker Present Absent
mCpG
OCT4
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Morphology
肠道样.tif
gut epithelial-like structure
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Morphology
SMA.tif
αSMA
软骨.tif
cartilage-like structure
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
Morphology
神经管.tif
neural tube-like structure

Microbiology / Virus Screening

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Passage number: 10
Karyotyping method: G-Banding

Other Genotyping (Cell Line)