Generation of two clonal iPSC lines, ICGi019-A and ICGi019-B, by reprogramming peripheral blood mononuclear cells of a patient suffering from hypertrophic cardiomyopathy and carrying a heterozygous p.M659I mutation in MYH7


Hypertrophic cardiomyopathy (HCM) is one of the most frequent cardiovascular diseases but no methods to prevent its progression have been developed. Cardiomyocytes derived from patient-specific induced pluripotent stem cells can become a platform to study pathogenesis of the disease and to search for more effective therapy methods. We generated two iPSC lines from peripheral blood mononuclear cells of an HCM patient with heterozygous p.M659I mutation in MYH7 using episomal vectors. The iPSC lines expressed pluripotency markers, demonstrated ability to spontaneously differentiate into derivatives of three germ layers, and retained the mutation. Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Dementyeva EV, Kovalenko VR, Zhiven MK, Ustyantseva EI, Kretov EI, Vyatkin YV, Zakian SM
Journal Stem cell research
Publication Date 2020 Jul;46:101840
PubMed 32422568
DOI 10.1016/j.scr.2020.101840

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