General

Cell Line

hPSCreg name SKLOi004-A
Cite as:
SKLOi004-A (RRID:CVCL_C9GU)
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
SKLOi004-B
Donor diseases:
Retinoblastoma
VRFi001-A
(VRF-RBiPSC01)
Donor diseases:
Retinoblastoma
LVPEIi002-A
(LVIP02-RB-1)
Donor's gene variants:
RB1
Donor diseases:
Retinoblastoma
MUi015-A
(RB1)
Donor diseases:
Retinoblastoma
Last update 24th August 2023
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Provider

Generator State Key Laboratory of Ophthalmology (SKLO)

External Databases

BioSamples SAMEA114299543
Cellosaurus CVCL_C9GU
Wikidata Q123033511

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • RB
  • retinoblastoma
  • RETINOBLASTOMA, MALIGNANT
  • Retinoblastoma
  • Retinoblastoma, NOS
show more synonyms

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA114299544

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. xuping7@mail2.sysu.edu.cn
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved?
Approval number
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A thin, transparent tissue divided into the palpebral conjunctiva (covering the inner side of the eye lid) and the bulbar conjunctiva (covering the eyeball).
Synonyms
  • CONJUNCTIVA
  • Conjunctiva
  • conjunctiva
  • conjunctivae
  • conjunctivas
  • tunica conjunctiva
  • wall of conjunctival sac
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Episomal

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Medium mTeSR™ Plus

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
TRA 1-60
Yes
SOX2
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
Differentiation Potency
Endoderm
Ont Id: NCIT_C12706
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Morphology
TUJ1

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal Karyotype
Karyotyping method: G-Banding

Other Genotyping (Cell Line)