RB1

General#

Cell Line

hPSCreg Name MUi015-A
Alternative name(s)
RB1
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 22nd March 2018
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Provider

Generator Mahidol University (MU)
Owner Mahidol University (MU)
Derivation country Thailand

External Databases

BioSamples SAMEA104271470
Cellosaurus CVCL_VE63

General Information

Is the cell line available in principle?
No

Donor Information#

General Donor Information

Sex male
Ethnicity Thai

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Retinoblastoma
The donor is a carrier of a disease-associated mutation and affected.
Disease associated phenotypes
  • Retinoblastoma
  • Burkitt lymphoma

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46,XY,del(13)(q14q22)
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes
Array CGH

External Databases (Donor)

BioSamples SAMEA104271471

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Age of donor (at collection) 15-19
Passage number reprogrammed P2

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Type of used vector free reprogramming factor(s)
mRNA

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
NANOG
Yes
TRA 1-81
Yes
SSEA-4
Yes
ZFP42 (REX-1)
Yes
DNMT3B
Yes
TDGF1
Yes
SOX2
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY, del(13)(q14q22)
Karyotyping method: G-Banding

Other Genotyping (Cell Line)