LCA.1.H.iPSC.1

General#

Cell Line

hPSCreg Name RIi010-A
Alternative name(s)
LCA.1.H.iPSC.1
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 8th December 2017
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Provider

Generator Royan Institute (RI)
Derivation country Iran

External Databases

Cellosaurus CVCL_C814

General Information

Publications
Is the cell line available in principle?
Yes

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Leber congenital amaurosis
The donor is affected.
Synonyms
  • Leber's disease
  • Leber congenital amaurosis type 13
  • Leber congenital amaurosis type 14
  • Leber's congenital amaurosis
  • LCA
  • Leber's amaurosis
show more synonyms

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.

Reprogramming method

Vector type Integrating
Vector Transposon ()
Genes
Is the used vector excisable?
Yes
Absence of reprogramming vector(s)?
Unkown
Reprogramming vectors silenced?

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Surface coating Matrigel/Geltrex
CO2 Concentration 5 %
Medium Other medium:
Base medium: DMEM F/12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-glutamine 2 mM
β-mercaptoethanol 0.1 mM
nonessential amino acid 1 %
penicillin 100 units/ml
streptomycin 100 µg/ml
insulin-transferrin-selenite
bFGF

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
SOX2
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY

Other Genotyping (Cell Line)