Generation of iPSC line (FMCPGHi003-A) from human PBMCs of a patient with Familial hemiplegic migraine type 3
Summary
Peripheral blood mononuclear cells (PBMCs) were obtained from a patient diagnosed with Familial Hemiplegic Migraine Type 3, who carried a heterozygous A > C mutation in the SCN1A gene and reprogrammed using CytoTuneTM-iPS 2.0 Sendai Reprogramming Kit. The iPSC line maintained the mutation while expressing markers of pluripotency. Additionally, it exhibited a normal karyotype and demonstrated potential for in vitro differentiation into cells representing all three embryonic germ layers. Copyright © 2024. Published by Elsevier B.V.
| Authors | Wang T, Li Y, Yang C, Yuan H, Na W, Yu S |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Sep;79:103465 |
| PubMed | 38880015 |
| DOI | 10.1016/j.scr.2024.103465 |