EP2C001 C4	
    			    			
                NUIGi060-C            
            
        General
Donor Information
General Donor Information | 
					|
| Sex | male | 
Phenotype and Disease related information (Donor) | 
					|
| Diseases | No disease was diagnosed.
																	 | 
					
Donor Relations | 
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| Other cell lines of this donor | |
External Databases (Donor) | 
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| BioSamples | SAMEA111562831 | 
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes | 
| Was the consent voluntarily given? | Yes | 
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes | 
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | Yes | 
| Do you (Depositor/Provider) hold the original Donor Consent Form? | Yes | 
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised | 
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes | 
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No | 
| How may genetic information associated with the cell line be accessed? | No information | 
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No | 
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes | 
| Name of accrediting authority involved? | Galway University Hospitals/NUIG Clinical and Research Ethics Committee | 
| Approval number | C.A.750 | 
| Do you have obligations to third parties in regard to the use of the cell line? | No | 
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No | 
hIPSC Derivation
General | 
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| Source cell line name | 
															F.EP2C001																										 Derived from same source line (potentially other lot and donor, see below):
							
							 
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| Source cell type | 
										
				
	
	
			 Synonyms 
		
  | 
							
Reprogramming method | 
						|
| Vector type | Non-integrating | 
| Vector | Sendai virus | 
Vector free reprogramming | 
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Other | 
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| Derived under xeno-free conditions | 
								 Unknown 							 | 
						
| Derived under GMP? | 
								 Unknown 							 | 
						
| Available as clinical grade? | 
								 Unknown 							 | 
						
Culture Conditions
| Medium | 
								Essential 8™ Flex		
		
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Characterisation
Analysis of Undifferentiated Cells
Morphology pictures
Differentiation Potency
In vitro spontaneous differentiation
					| Marker | Expressed | 
| Class III beta tubulin (homo sapiens) | 
										 Yes 					                 | 
								
Morphology
Genotyping
Karyotyping (Cell Line) | 
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| Has the cell line karyotype been analysed? | 
								 Yes 								
																	
												Normal
											 
																				
																															
											Passage number: 20											 
																															
																			 | 
						
Other Genotyping (Cell Line) | 
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