Establishment of PLAFMCi004-A induced pluripotent stem cells derived from PBMCs from a healthy individual
Summary
Peripheral blood mononuclear cells (PBMCs) were isolated from a healthy male individual. PBMCs were reprogrammed into human induced pluripotent stem cells (iPSCs) using plasmids carrying the following reprogramming factors: NANOG, LIN28, OCT4, SOX2, c-MYC, and KLF4. This cell line expressed pluripotency markers, exhibited the normal male karyotype (46, XY), and had the potential to differentiate into 3 germ layers, as confirmed by techniques such as flow cytometry. The iPSC obtained from a healthy individual can be used for organoids to reveal developmental mechanism of tissue and organ regeneration, stem cell therapy and drug screening. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Shang S, Chen K, Cai G, Bai X, Chen X, Li Q |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2021 May;53:102316 |
| PubMed | 33878710 |
| DOI | 10.1016/j.scr.2021.102316 |