iPS_LGH_AHDC1_P3
USPi003-A
General
Cell Line |
|
| hPSCreg name | USPi003-A |
| Cite as: | USPi003-A (RRID:CVCL_C6TF) |
| Alternative name(s) |
iPS_LGH_AHDC1_P3
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
IMBAi017-A (B002-ARID1B#8, Pat.2 ARID1B+/- clone 2a (XX), Pat.2 ARID1B+/- clone 2b (XX)) Donor diseases: Coffin-Siris Syndrome |
| Last update | 22nd February 2023 |
| Notes | AHDC1(NM_001371928.1):c.1481__1482del(p.Lys494SerfsTer22) Xia-Gibbs syndrome model |
| User feedback | |
Provider |
|
| Generator | Universidade de São Paulo (USP) |
| Owner | Laboratory of Human Genetics - Institute of Biosciences (CEGH) |
| Distributors | |
| Derivation country | Brazil |
External Databases |
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| BioSamples | SAMEA111506901 |
| Cellosaurus | CVCL_C6TF |
| Wikidata | Q117704949 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Additional restrictions:
To discuss research collaborations, send an email to Dr. Ana Krepischi (ana.krepischi@ib.usp.br) |
Donor Information
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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Karyotyping (Donor) |
|
| Has the donor karyotype been analysed? |
Yes
46,XY
Karyotyping method:
Molecular karyotyping by SNP array
http:// |
External Databases (Donor) |
|
| BioSamples | SAMEA111506902 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | Yes |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | Yes |
| Alternatives to consent are available? | No |
| Is there other documentation provided to the donor for consenting purposes? | No |
| Confirm that consent was obtained by a qualified professional | Yes |
| Has the donor agreed to be re-contacted? | Yes |
| Has the donor been informed about how her/his data will be protected? | Yes |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | No |
| Does consent expressly prevent the derivation of pluripotent stem cells? | No |
| Does consent pertain to a specific research project? | Yes |
| Details on restriction to research project | Research project of the Laboratory of Human Genetics (USP). Project theme: ethilogial bases of intellectual disability; involves genomic and functional investigation |
| Does consent permit unforeseen future research, without further consent? | Yes |
| Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? | No |
| Does consent expressly prevent development of commercial products? | No |
| Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? | Yes |
| Does consent expressly permit storage of donated embryo/tissue for an unlimited time? | Yes |
| Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? | Yes |
| Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
Does consent permit research by | |
| an academic institution? | Yes |
| a public organisation? | Yes |
| a non-profit company? | Yes |
| a for-profit corporation? | Yes |
| Does consent expressly permit collection of genetic information? | Yes |
| Does consent expressly permit storage of genetic information? | Yes |
| Does consent prevent dissemination of genetic information? | No |
| Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? | No |
| Has the donor consented to receive information discovered during use of donated embryo/tissue that has significant health implications for the donor? | Yes |
| How may genetic information associated with the cell line be accessed? | Controlled Access |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? | Yes |
| Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? | Yes |
| Does consent permit access to medical records of the donor? | Yes |
| Please describe how access is provided: | Contact Dr. Ana Krepischi (ana.krepischi@ib.usp.br) |
| Does consent permit access to any other source of information about the clinical treatment or health of the donor? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Comitê de Ética em Pesquisa - Seres Humanos (CEP) do Instituto de Biociências da USP |
| Approval number | CEP_2589398 |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? | Yes |
| Name of accrediting authority involved? | Comitê de Ética em Pesquisa - Seres Humanos (CEP) do Instituto de Biociências da USP |
| Approval number | CEP_2589398 |
| Do you have obligations to third parties in regard to the use of the cell line? | No |
| Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? | No |
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | Addgene |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation
General |
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| Source cell type |
A sample of peripheral blood mononuclear cells.
Synonyms
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| Source cell origin |
Blood drawn from a limb.
Synonyms
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Episomal |
| Is reprogramming vector detectable? |
Yes |
| Methods used |
PCR
|
| Notes on reprogramming vector detection | The cells were reprogrammed using plasmids pCXLE-hOCT3/4-shp53, pCXLE-hSK, and pCXLE-hUL, containing the transcription factors OCT3/4, SOX2, KLF4, L-MYC, and LIN28, in addition to a shRNA for p53 suppression. The cells were tested for plasmid integration in to the genome by convetional PCR applied to OriP and EBNA-1 loci, with negative results. |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Medium |
Essential 8™
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| SOX2 |
Yes |
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| SSEA-4 |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| NANOG |
Yes |
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Differentiation Potency
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46,XY
Passage number: 8
Karyotyping method:
Array CGH
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Other Genotyping (Cell Line) |
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