AYC12, HKUi002-A-AYC12

General#

Cell Line

hPSCreg Name HKUi002-A
Alternative name(s)
AYC12, HKUi002-A-AYC12
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 4th September 2020
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Provider

Generator The University of Hong Kong (HKU)
Derivation country Hong Kong

External Databases

BioSamples SAMEA6880596
Cellosaurus CVCL_ZA51

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: allowed
Additional restrictions:

Only if it is related to collaborated research partnership

Donor Information#

General Donor Information

Sex male
Ethnicity Chinese

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
dilated cardiomyopathy (primary disease)
X-linked dilated cardiomyopathy
The donor is not a carrier of a disease-associated mutation and affected.
Stage
Dilated cardiomyopathy with heart failure, post-heart transplantation
Genetic variants
cytoband location: Xp21.2-21.1
c.31 + 1G > A (Ref. sequence: NM_004006.2)
Heterozygous
J Pediatr Neurol 2018; 16(01): 029-034 DOI: 10.1055/s-0037-1603997
Disease associated phenotypes
  • heart failure from dilated cardiomyopathy
Family history Nil
Is the medical history available upon request? Yes. The patient had significant heart failure due to dilated cardiomyopathy required heart transplantation. Further medical information will be available upon request.
Is clinical information available? Yes. Clinically patient had no major skeletal muscle weakness. Creatine kinase level was normal initially but raised few years after transplantation. Further clinical information will be available upon request.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46XY
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA6880597

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? Yes
If you do not hold the SIGNED Donor Consent Form, have you obtained a copy of the unsigned Donor Consent Form from the holder? No
Alternatives to consent are available? No
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? Yes
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent pertain to a specific research project? Yes
Details on restriction to research project Generation of patient-derived induced pluripotent stem cells for differentiation to cardiomyocytes and skeletal muscles as disease model to study the transcription factors and gene editing
Does consent permit unforeseen future research, without further consent? Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent expressly prevent development of commercial products? No
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? Yes
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? No
Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? No
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? No
a non-profit company? No
a for-profit corporation? No
Does consent expressly permit collection of genetic information? Yes
Does consent expressly permit storage of genetic information? Yes
Does consent prevent dissemination of genetic information? No
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? Yes
Has the donor consented to receive information discovered during use of donated embryo/tissue that has significant health implications for the donor? No
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? No
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? No
Does consent permit access to medical records of the donor? Yes
Please describe how access is provided: Through the hospital electronic medical health record system
Does consent permit access to any other source of information about the clinical treatment or health of the donor? Yes
Contact data, institution, or website: Through regular review of the update progress of patient's health and treatment from the hospital electronic medical health record system
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Hong Kong University/ Hospital Authority Hong Kong West Cluster Institutional Review Board
Approval number UW-17274
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Hong Kong University/ Hospital Authority Hong Kong West Cluster Institutional Review Board
Approval number UW-17274
Do you have obligations to third parties in regard to the use of the cell line? No
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? CytoTune™-iPS 2.0 Sendai Reprogramming Kit (Invitrogen)
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation#

General

Source cell type
mononuclear cell
A leukocyte with a single non-segmented nucleus in the mature form.
Synonyms
  • mononuclear leukocyte
Source cell origin
blood
Bodily fluid in animals that delivers necessary substances such as nutrients and oxygen to the cells and transports metabolic waste products away.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
Yes
Methods used
RT-PCR

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones The clones were manually picked if they showed large nuclei and grow in flat colonies with an embryonic stem cell (ESC)-like morphology
Derived under xeno-free conditions
Yes
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Score:
Marker Present Absent
mCpG
OCT4
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)