KSCBi002-B-1

hFmiPS2

General#

Cell Line

hPSCreg Name KSCBi002-B-1
Alternative name(s)
hFmiPS2
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 20th November 2017
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator National Stem Cell Bank (KSCB)

External Databases

BioSamples SAMEA104012355
Cellosaurus CVCL_IT65

General Information

Publications
Is the cell line available in principle? Available with restrictions: Available for non-commercial research
Subclone of

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104012353

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

The source cell information can be found in the parental cell line KSCBi002-B.

Reprogramming method

Vector type Non-integrating
Vector modified RNA
Genes
Is reprogramming vector detectable?
No
Methods used
Immunostaining, PCR, RT-PCR

Vector free reprogramming

Type of used vector free reprogramming factor(s)
mRNA
mRNA

Other

Selection criteria for clones under the checking of cell morphology by phase contrast microscopy
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Gelatin
Feeder cells STO
Passage method Enzymatically
Dispase
CO2 Concentration 5 %
Medium Other medium:
Base medium: DMEM/F12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
MEM-NEAA 0.1 mM
beta-mercaptoethanol 0.1 mM
bFGF 4 ng/ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Differentiation Potency
AFP
Ont Id: OMIT_0030150
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
Brachyury
Yes
PAX6
Ont Id: OMIT_0034078
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#