Generation of human induced pluripotent stem cell lines from human dermal fibroblasts using a modified RNA system


We generated human induced pluripotent stem cells (KSCBi002-B and KSCBi002-B-1) from the dermal fibroblasts of a donor using a modified RNA-based gene delivery method. According to GTG-banding analysis, the generated KSCBi002-B line has a cytogenetic abnormality (46,XY, t(1;4)(q21;q25)) that is distinct from that of the donor, whereas KSCBi002-B-1 has a normal karyotype (46,XY). These cell lines can be useful as a model for characterizing the hiPSCs generated by a non-viral and non-integrative system, or as a chromosomal balanced translocation model. These two cell lines are registered and available from the National Stem Cell Bank, Korea National Institute of Health. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Uhm KO, Go GY, Kim SJ, Jo EH, Choi HY, Im YS, Ha HY, Kim JH, Koo SK
Journal Stem cell research
Publication Date 2017 Oct;24:148-150
PubMed 29034882
DOI 10.1016/j.scr.2017.02.009

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