BGU01iPORhet

General

Cell Line

hPSCreg name BGUi001-A
Cite as:
BGUi001-A (RRID:CVCL_A9XC)
Alternative name(s)
BGU01iPORhet
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
BGUi002-A
(BGU02iPOR)
Donor diseases:
P450 Oxidoreductase Deficiency
BGUi003-A
(BGU03iPOR)
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P450 Oxidoreductase Deficiency
UNIZARi001-A
(FiPSTK2-2)
Donor's gene variants:
TK2, TK2
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Mitochondrial DNA depletion syndrome, myopathic form
ZZUi030-A
(ZZU-iPS-SPG7-001)
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Spastic paraplegia type 7
UTSWi001-A
(FA1)
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Friedreich Ataxia
UPITTi004-A
(CN090 C5A5J2)
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Sickle cell anemia
UPITTi004-B
(CN090 C1B5B5)
Donor diseases:
Sickle cell anemia
FRIMOi004-A
(STGD2_ FiPS4F1.7)
Donor diseases:
Stargardt Disease
HIHDNDi001-A
(A30P-3, SNCA3, Tue_020_A)
Donor's gene variants:
SNCA, SNCA, SNCA
Donor diseases:
autosomal dominant Parkinson disease 1
BIHi276-A
Donor's gene variants:
MT-ATP6
Donor diseases:
Leigh Disease
BIHi267-B
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MT-ATP6
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Leigh Disease
VUi011-A
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LUMCi002-A
(113-6, LUMC0113iATAX06)
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Spinocerebellar Ataxia Type 1
HIHDNDi001-B
(A30P-4, SNCA4, Tue_020_B)
Donor's gene variants:
SNCA, SNCA, SNCA
Donor diseases:
autosomal dominant Parkinson disease 1
LUMCi002-B
(113-7, LUMC0113iATAX07)
Donor diseases:
Spinocerebellar Ataxia Type 1
LUMCi002-C
(113-8, LUMC0113iATAX08)
Donor diseases:
Spinocerebellar Ataxia Type 1
ZZUi026-A
(ZZU-iPS-SCA3-003)
Donor diseases:
Spinocerebellar Ataxia Type 3
DHMi004-A
(HOS_1460)
Donor diseases:
Holt-Oram Syndrome
MDCi007-A
(8993-A12)
Donor diseases:
Leigh Disease
MDCi008-A
(8993-B12)
Donor diseases:
Leigh Disease
MDCi010-A
(8993-D7)
Donor diseases:
Leigh Disease
HDZi003-A
(hiPSC NP0038)
Donor's gene variants:
TMEM43
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arrhythmogenic right ventricular dysplasia 5
CTGUi001-A
(FD01, F01)
Donor diseases:
Fabry Disease
UKWNLi007-A
(GLA-515G>A-1, GLA-C172Y-iPSC-1, FD1210/1)
Donor diseases:
Fabry Disease
AIBNi015-A
(SPG1-AU01C15)
Donor diseases:
hereditary spastic paraplegia 56
TNRMCi001-A
(iTAF32)
Donor's gene variants:
CFTR
Donor diseases:
Cystic fibrosis
UKBi014-A
(A-257s2)
Donor diseases:
Walker-Warburg syndrome
Last update 22nd January 2021
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Provider

Generator Ben Gurion University of the Negev (BGU)
Owner Ben Gurion University of the Negev (BGU)
Distributors
Derivation country Israel

External Databases

Cellosaurus CVCL_A9XC
BioSamples SAMEA7873753
Wikidata Q102113614

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex male
Ethnicity Bedouin

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • P450 Oxidoreductase Deficiency

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Karyotyping method: G-Banding

External Databases (Donor)

BioSamples SAMEA7873752

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. Prof Eli Hershkovitz, Soroka medical center
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information Prof Eli Hershkovitz, Soroka medical center
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Medium Other medium:
Base medium: Nutristem
Main protein source:
Serum concentration: %

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
SOX2
Yes
SSEA-4
Yes
TRA 1-60
Yes
OCT3/4
Yes
Score:
Marker Present Absent
mCpG
OCT4
Embryoid bodies were generated and spontaneously differentiated for 21 days, after which the cells were stained for 68 kDa Neurofillament (ectoderm), α-Fetoprotein (endoderm), α-smooth muscle actin (mesoderm).
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Actin, Aortic Smooth Muscle
Ont Id: NCIT_C103972
In vitro spontaneous differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal, male
Passage number: 10
Karyotyping method: G-Banding

Other Genotyping (Cell Line)