3053

General#

Cell Line

hPSCreg Name
NOVOe001-A
Alternative name(s)
3053
Cell line type Human embryonic stem cell (hESC)
Last update 22nd June 2021
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Novo Nordisk A/S (NOVO)
Owner Novo Nordisk A/S (NOVO)
Derivation country Sweden

External Databases

BioSamples SAMEA9232316
CLO CLO_0103345

General Information

Projects
* Is the cell line readily obtainable for third parties?
No

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

External Databases (Donor)

BioSamples SAMEA9232317

Ethics#

Was the embryo established purely for research purposes? No
Have both parents consented to the use of the embryo for ESC derivation? Yes
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? No
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethical Committee Gothenburg
Approval number 411-14

hESC Derivation#

Date of derivation 2014-10-10
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No

Culture Conditions#

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
OCT4
Yes
SOX2
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
Marker Expressed
SOX17
Yes
PDX1
Yes
NKX6.1
Unknown
Cardiac Muscle Cell
Ont Id: CL_0000746
Marker Expressed
NKX2.5
Yes
TNNT2
Yes
MYH6
Yes
Neuroepithelial Stem Cell
Ont Id: CL_0002259
Marker Expressed
Nestin
Yes
Tubulin Beta 3 Class III
Yes
PAX6
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX. Chromosome analysis of the fixed cell suspension from this stem cell line has shown an apparently normal female karyotype in 20 cells examined. The preparations obtained from this sample were of sufficient quality to detect numerical and large structural abnormalities.
Passage number: 12
Karyotyping method: G-Banding

Other Genotyping (Cell Line)