General

Cell Line

hPSCreg Name
MUi032-A
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 27th September 2022
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Provider

Generator Mahidol University (MU)

External Databases

Cellosaurus CVCL_B7E4
BioSamples SAMEA111372278
Wikidata Q112930141
CLO CLO_0104069

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Choroideremia
The donor is affected.
Synonyms
  • Choroideremia
  • Progressive Choroidal Atrophy

External Databases (Donor)

BioSamples SAMEA111396673

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Mahidol University Central Institutional Review Board (MU-CIRB)
Approval number MU-CIRB 2019/316.0912
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell line name hematopoietic stem cells
Derived from same source line (potentially other lot and donor, see below):
Source cell type
Hematopoietic Stem Cell
Primitive blood cells derived from embryonic mesenchyme capable of differentiating into any of the blood cell line progenitor cells (erythroblasts, young granulocytic series cells, megakaryocytes, etc.)
Synonyms
  • Hematopoietic Progenitor Cell
  • Ferrata cell
  • Hemohistioblast
  • Hematohistioblast
  • Hematopoietic Stem Cell
  • HSC
  • HSCs
  • HPC
  • Hematopoietic Progenitor Cells
  • Hematopoietic Stem Cells
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
SSEA-4
Yes
TRA 1-60
Yes
POU5F1 (OCT-4)
Yes
TDGF1
Yes
DNMT3B
Yes
SOX2
Yes
GDF3
Yes
GABRB3
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
FOXA2
Yes
Sox17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
TBX6
Yes
TBXT
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
NEUROD1
Yes
TUJ1
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal male karyotype (46, XY)
Karyotyping method: G-Banding

Other Genotyping (Cell Line)