HIHCNi008-A-4

iPSC-REEP1_hetKO2

The cell line is not validated yet.

General

Cell Line

hPSCreg name HIHCNi008-A-4
Cite as:
HIHCNi008-A-4 (RRID:CVCL_D5HA)
Alternative name(s)
iPSC-REEP1_hetKO2
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
HIHCNi008-A
(iPSC-CO-4)
HIHCNi008-A-3
(iPSC-REEP1_homKO2)
Last update 30th January 2024
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Provider

Generator Hertie Institute for Clinical Brain Research - Clinical Neurogenetics (HIHCN)

External Databases

BioSamples SAMEA115125887
Cellosaurus CVCL_D5HA
Wikidata Q127381755

General Information

Publications
* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46XY

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA115124423

Ethics

Also have a look at the ethics information for the parental line HIHCNi008-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line HIHCNi008-A.

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Medium Essential 8™

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
TRA 1-81
Yes
NANOG
Yes
SSEA-3
Yes
SOX2
Yes
OCT4
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
FOXA2
Yes
SOX17
Yes
Morphology
Smooth Muscle Cell
Ont Id: CL_0000192
In vitro spontaneous differentiation
Morphology
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Morphology

Genotyping

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Synonyms
  • SPG31
  • autosomal dominant spastic paraplegia 31
  • autosomal dominant spastic paraplegia type 31
Genetic modifications
Gene knock-out
22p11.2
CRISPR-associated (CRISPR/Cas) System