Derivation of human induced pluripotent stem cell line (HUSTi001-A) from a 40-year-old patient with idiopathic infertility


Human fibroblasts were isolated from foreskin of a clinically diagnosed 40-year old patient with idiopathic infertility. The fibroblasts were reprogrammed with the Yamanaka KOSM transcriptional factors using the retroviral vectors. The obtained induced pluripotent stem cell (iPSC) line showed pluripotency verified by the expression of pluripotency markers, NANOG, SOX2, OCT4, TRA-1-60, and SSEA-4. And the iPSC line was demonstrated to have the three germ layers differentiation capacity in vivo by teratoma assay. The iPSC line also showed normal karyotype. This patient-specific iPSC line can be used to explore the mechanism for idiopathic male infertility. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Fang F, Li Z, Zhao Q, Ye Z, Zhao K, Gu X, Shen S, Reijo Pera RA, Xiong C
Journal Stem cell research
Publication Date 2018 Mar;27:136-139
PubMed 29414604
DOI 10.1016/j.scr.2018.01.026

Research Projects

Cell Lines