Generation of two induced pluripotent stem cell lines (FAMRCi005-A and FAMRCi005-B) from patient carrying genetic variant LMNA p.Asp357Val


LMNA mutations are often linked to laminopathies characterized by tissue-specific disorders. We generated two induced pluripotent stem cells lines from patient carrying genetic variant LMNA p.Asp357Val associated with paroxysmal ventricular tachycardia and myopathy. Reprogramming of patient's peripheral blood mononuclear cells was performed using Sendai viruses. Characterization of the FAMRCi005-A and FAMRCi005-B lines revealed that generated iPSC lines expressed pluripotent stem cell markers, had normal karyotype and demonstrated triliniage differentiation ability. Generated cell lines can be used to investigate the molecular links between LMNA genetic variants and cardiac disorders. Copyright © 2020. Published by Elsevier B.V.

Authors Klauzen P, Perepelina K, Khudiakov A, Zlotina A, Fomicheva Y, Pervunina T, Vershinina T, Kostareva A, Malashicheva A
Journal Stem cell research
Publication Date 2020 Mar;43:101719
PubMed 32062135
DOI 10.1016/j.scr.2020.101719

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